Transplants of neurosphere cell suspensions from aged mice are functional in the mouse model of Parkinson's.
Identifieur interne : 002952 ( Main/Exploration ); précédent : 002951; suivant : 002953Transplants of neurosphere cell suspensions from aged mice are functional in the mouse model of Parkinson's.
Auteurs : Kelly K. Meissner [Canada] ; David L. Kirkham ; Laurie C. DoeringSource :
- Brain research [ 0006-8993 ] ; 2005.
English descriptors
- KwdEn :
- Aging (physiology), Amphetamine (pharmacology), Animals, Apomorphine (pharmacology), Astrocytes (physiology), Behavior, Animal (drug effects), Behavior, Animal (physiology), Behavioral Symptoms (therapy), Corpus Striatum (cytology), Corpus Striatum (surgery), Disease Models, Animal, Dopamine Agonists (pharmacology), Dopamine Uptake Inhibitors (pharmacology), Functional Laterality, Glial Fibrillary Acidic Protein (metabolism), Green Fluorescent Proteins (biosynthesis), Green Fluorescent Proteins (genetics), Mice, Mice, Inbred C57BL, Mice, Transgenic, Neurofilament Proteins (metabolism), Neurons (physiology), Oxidopamine (toxicity), Parkinson Disease (complications), Parkinson Disease (etiology), Parkinson Disease (therapy), Stem Cell Transplantation (methods), Time Factors, Tyrosine 3-Monooxygenase (metabolism).
- MESH :
- chemical , biosynthesis : Green Fluorescent Proteins.
- chemical , genetics : Green Fluorescent Proteins.
- chemical , metabolism : Glial Fibrillary Acidic Protein, Neurofilament Proteins, Tyrosine 3-Monooxygenase.
- chemical , pharmacology : Amphetamine, Apomorphine, Dopamine Agonists, Dopamine Uptake Inhibitors.
- complications : Parkinson Disease.
- cytology : Corpus Striatum.
- drug effects : Behavior, Animal.
- etiology : Parkinson Disease.
- methods : Stem Cell Transplantation.
- physiology : Aging, Astrocytes, Behavior, Animal, Neurons.
- surgery : Corpus Striatum.
- therapy : Behavioral Symptoms, Parkinson Disease.
- chemical , toxicity : Oxidopamine.
- Animals, Disease Models, Animal, Functional Laterality, Mice, Mice, Inbred C57BL, Mice, Transgenic, Time Factors.
Abstract
Neural stem cell therapy has the potential to treat neurodegenerative disorders. For Parkinson's disease (PD), the goal is to enhance the dopamine system sufficiently to restore the control of movement and motor activities. In consideration of autologous stem cell therapy for PD, it will be necessary to propagate the cells in most cases from aged brain tissue. We isolated cells from the subventricular zone (SVZ) in the brains of 1-year-old enhanced green fluorescent protein (GFP) mice and generated neurospheres in culture. Neurospheres yielding high numbers of neurons and astrocytes "de novo" were selected and cryopreserved before evaluating the efficacy of neurosphere cell suspensions transplanted to the 6-hydroxydopamine (6-OHDA) model of PD. In mice unilaterally lesioned with 6-OHDA, transplants of neurosphere cell suspensions to the striatum yielded astrocytes and tyrosine hydroxylase positive neurons that reduced or reversed the drug-induced behavioral circling response to amphetamine and apomorphine. Control mice without the cell suspensions showed no change in the motor behavior. Our results indicate that the SVZ in the aged mouse brain contains cells that can be expanded in the form of neurospheres, cryopreserved, re-expanded and then transplanted into the damaged dopamine system to generate functional cell progeny that offset the motor disturbances in the nigrostriatal system.
DOI: 10.1016/j.brainres.2005.07.057
PubMed: 16140285
Affiliations:
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Meissner</name><affiliation wicri:level="4"><nlm:affiliation>Department of Pathology and Molecular Medicine, Health Science Center, HSC 1R1, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5.</nlm:affiliation><country>Canada</country><wicri:regionArea>Department of Pathology and Molecular Medicine, Health Science Center, HSC 1R1, McMaster University, 1200 Main Street West, Hamilton, Ontario</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Kirkham, David L" sort="Kirkham, David L" uniqKey="Kirkham D" first="David L" last="Kirkham">David L. Kirkham</name></author><author><name sortKey="Doering, Laurie C" sort="Doering, Laurie C" uniqKey="Doering L" first="Laurie C" last="Doering">Laurie C. 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Meissner</name><affiliation wicri:level="4"><nlm:affiliation>Department of Pathology and Molecular Medicine, Health Science Center, HSC 1R1, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5.</nlm:affiliation><country>Canada</country><wicri:regionArea>Department of Pathology and Molecular Medicine, Health Science Center, HSC 1R1, McMaster University, 1200 Main Street West, Hamilton, Ontario</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Kirkham, David L" sort="Kirkham, David L" uniqKey="Kirkham D" first="David L" last="Kirkham">David L. Kirkham</name></author><author><name sortKey="Doering, Laurie C" sort="Doering, Laurie C" uniqKey="Doering L" first="Laurie C" last="Doering">Laurie C. Doering</name></author></analytic><series><title level="j">Brain research</title><idno type="ISSN">0006-8993</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aging (physiology)</term><term>Amphetamine (pharmacology)</term><term>Animals</term><term>Apomorphine (pharmacology)</term><term>Astrocytes (physiology)</term><term>Behavior, Animal (drug effects)</term><term>Behavior, Animal (physiology)</term><term>Behavioral Symptoms (therapy)</term><term>Corpus Striatum (cytology)</term><term>Corpus Striatum (surgery)</term><term>Disease Models, Animal</term><term>Dopamine Agonists (pharmacology)</term><term>Dopamine Uptake Inhibitors (pharmacology)</term><term>Functional Laterality</term><term>Glial Fibrillary Acidic Protein (metabolism)</term><term>Green Fluorescent Proteins (biosynthesis)</term><term>Green Fluorescent Proteins (genetics)</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Mice, Transgenic</term><term>Neurofilament Proteins (metabolism)</term><term>Neurons (physiology)</term><term>Oxidopamine (toxicity)</term><term>Parkinson Disease (complications)</term><term>Parkinson Disease (etiology)</term><term>Parkinson Disease (therapy)</term><term>Stem Cell Transplantation (methods)</term><term>Time Factors</term><term>Tyrosine 3-Monooxygenase (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Green Fluorescent Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Green Fluorescent Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glial Fibrillary Acidic Protein</term><term>Neurofilament Proteins</term><term>Tyrosine 3-Monooxygenase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Amphetamine</term><term>Apomorphine</term><term>Dopamine Agonists</term><term>Dopamine Uptake Inhibitors</term></keywords><keywords scheme="MESH" qualifier="complications" xml:lang="en"><term>Parkinson Disease</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Corpus Striatum</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Behavior, Animal</term></keywords><keywords scheme="MESH" qualifier="etiology" xml:lang="en"><term>Parkinson Disease</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Stem Cell Transplantation</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Aging</term><term>Astrocytes</term><term>Behavior, Animal</term><term>Neurons</term></keywords><keywords scheme="MESH" qualifier="surgery" xml:lang="en"><term>Corpus Striatum</term></keywords><keywords scheme="MESH" qualifier="therapy" xml:lang="en"><term>Behavioral Symptoms</term><term>Parkinson Disease</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Oxidopamine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Disease Models, Animal</term><term>Functional Laterality</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Mice, Transgenic</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Neural stem cell therapy has the potential to treat neurodegenerative disorders. For Parkinson's disease (PD), the goal is to enhance the dopamine system sufficiently to restore the control of movement and motor activities. In consideration of autologous stem cell therapy for PD, it will be necessary to propagate the cells in most cases from aged brain tissue. We isolated cells from the subventricular zone (SVZ) in the brains of 1-year-old enhanced green fluorescent protein (GFP) mice and generated neurospheres in culture. Neurospheres yielding high numbers of neurons and astrocytes "de novo" were selected and cryopreserved before evaluating the efficacy of neurosphere cell suspensions transplanted to the 6-hydroxydopamine (6-OHDA) model of PD. In mice unilaterally lesioned with 6-OHDA, transplants of neurosphere cell suspensions to the striatum yielded astrocytes and tyrosine hydroxylase positive neurons that reduced or reversed the drug-induced behavioral circling response to amphetamine and apomorphine. Control mice without the cell suspensions showed no change in the motor behavior. Our results indicate that the SVZ in the aged mouse brain contains cells that can be expanded in the form of neurospheres, cryopreserved, re-expanded and then transplanted into the damaged dopamine system to generate functional cell progeny that offset the motor disturbances in the nigrostriatal system.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Ontario</li></region><settlement><li>Hamilton (Ontario)</li></settlement><orgName><li>Université McMaster</li></orgName></list><tree><noCountry><name sortKey="Doering, Laurie C" sort="Doering, Laurie C" uniqKey="Doering L" first="Laurie C" last="Doering">Laurie C. Doering</name><name sortKey="Kirkham, David L" sort="Kirkham, David L" uniqKey="Kirkham D" first="David L" last="Kirkham">David L. Kirkham</name></noCountry><country name="Canada"><region name="Ontario"><name sortKey="Meissner, Kelly K" sort="Meissner, Kelly K" uniqKey="Meissner K" first="Kelly K" last="Meissner">Kelly K. Meissner</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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